Personajes mejorados: Gladiator, Green Arrow, Killer Croc, Poison Ivy, Atom Smasher, Catman, Dazzler, Deadshot, Solomon Grundy, Vixen.
Algunas de las nuevas características incluyen la nueva resolucion (800x600) con el screenpack actualizado, y la ventana redimensionable.
- Adam Warlock (Marvel)
- Alien (Especial)
- Agent Orange (Larfleeze) (DC)
- Binary/Binaria (clon)(Marvel)
- Black Manta (DC)
- Black Vulcan/Volcan Negro (Superamigos)(DC)
- Bloodsport (clon)(DC)
- Bonebreaker/Rompehuesos (Cosechadores)(Marvel)
- Conan (Especial)
- Crimson Dynamo/Dinamo Escarlata (Marvel)
- Doc Samson (Marvel)
- Falcon/Halcon (Marvel)
- General Zod (DC)
- Gamora (Marvel)
- Golem (Especial)
- Hawkwoman/Mujer Halcon (DC)
- Heimdall (Especial/Marvel)
- Hellcat/Gata Infernal (Marvel)
- Huntress/Cazadora (DC)
- Killer Frost/Hielo Mortal (DC)
- Kraven the Hunter (Marvel)
- Mockingbird/Pajaro Burlon (Marvel)
- Nemesis (Especial)
- Man Beast/Hombre Bestia (4btns)(Marvel)
- Mantis (Marvel)
- Moon Dragon/Dragon Lunar (Marvel)
- Mysterio (Marvel)
- Owlman/Hombre Buho (Sindicato del Crimen)(Nite Owl/Buho Nocturno) (DC)
- Pantha (clon)(DC)
- Penguin/Pinguino (DC)
- Red Arrow/Flecha Roja (Speedy/Veloz) (DC)
- Rick Flag (Escuadron Suicida)(DC)
- Riptide/Marea (Merodeadores)(4btns)(Marvel)
- Rocket Raccon/Mapache Cohete (Marvel)
- Scorpion/Escorpion (Marvel)
- Squirrel Girl/Chica Ardilla (Marvel)
- Starlord (Marvel)
- Star Sapphire/Zafiro Estelar (DC)
- Swamp Thing/Cosa del Pantano (DC)
- Thorn/Espina (DC)
- Thunderbird/Ave de Trueno (X-Men)(Marvel)
- Toyman/Juguetero (DC)
- Unknown Soldier (Escuadron Suicida)(DC)
- Ursa (DC)
- Valkyrie/Valquiria (Defensores)(Marvel)
- White Canary/Canario Blanco (DC)
- Winter Soldier/Soldado de Invierno (Marvel)
- Zombie (Especial)
Descargar Mugen Superheroes 2000 v4.5 y mas información.